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Category:Indian films Category:1993 films Category:1990s Hindi-language films Category:Films directed by Mahesh Bhatt Category:Films scored by Anand–Milind Category:Indian comedy films5d420d4119daa4dae70ec15d7ca6fc93 *tests/data/fate/vsynth2-dnxhd-hr-15.dnxhd 120872 tests/data/fate/vsynth2-dnxhd-hr-15.dnxhd 9bfbab7b2226fd49dcec5b7d2d1486a3 *tests/data/fate/vsynth2-dnxhd-hr-15.out.rawvideo stddev: 0.00 PSNR:999.99 MAXDIFF: 0 bytes: 1058400/ 1058400 In vitro diagnosis of acute appendicitis using real-time PCR. Acute appendicitis is a common surgical emergency with an incidence of approximately 300,000 new cases per year in the United States. A rapid diagnosis would allow early institution of therapy and potentially decrease the morbidity and mortality associated with this disorder. It has been reported that the presence of nucleic acid material in the peritoneal fluid of acute appendicitis patients can be detected by polymerase chain reaction (PCR). In this study, real-time PCR technology was utilized to evaluate the presence of mRNA transcripts in appendectomized tissues obtained from patients. Approximately 1 mg of total RNA was extracted from homogenized tissue. A 161-bp, cDNA-specific segment of the ovis aries IAPP gene was amplified with gene-specific primers. In vitro transcribed RNA (IVT) was used as a positive control. The IVT control served as a quantitative standard against which cDNA-specific PCR products were quantitated. A real-time PCR was carried out in a thermal cycler to determine the amplification efficiency of the cDNA-specific PCR. The same reaction was used to evaluate the tissue samples. Fifty-three appendectomy specimens were examined for the presence of mRNA transcripts. The mRNA was detected in 85% of the appendectomized tissues examined. These data indicate that real-time PCR is a sensitive and specific method for the detection of mRNA transcripts in tissues. It has the potential to serve

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